Table of Contents

Chapter 1: What Is Biotechnology?

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• Definition and History

• Fields of Application (Medicine, Agriculture, Industry)

• Traditional vs Modern Biotechnology

• Bioethics Introduction

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Chapter 2: Tools of Biotechnology

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• Restriction Enzymes

• PCR (Polymerase Chain Reaction)

• Gel Electrophoresis

• CRISPR (Intro Level Only)

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Chapter 3: Genetic Engineering

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• Gene Cloning

• Recombinant DNA

• Genetically Modified Organisms (GMOs)

• Gene Therapy Basics

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Chapter 4: Biotechnology in Medicine

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• Insulin Production Using Bacteria

• Vaccines and Antibodies

• Personalized Medicine

• Cancer Detection and Treatment

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Chapter 5: Biotechnology in Agriculture

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• GMO Crops (e.g., pest resistance)

• Golden Rice and Biofortification

• Cloning in Livestock

• Soil Microbes and Crop Health

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Chapter 6: Biotechnology in Industry

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• Using Microbes in Food and Drink

• Bioplastics and Enzymes in Detergents

• Fermentation and Biofuels

• Bioremediation

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Chapter 7: Applied Biology in Forensics

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• DNA Fingerprinting

• Analyzing Biological Evidence

• Paternity Testing

• Ethics in Genetic Privacy

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Chapter 8: Applied Biology in Environmental Science

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• Bioindicators and Biosensors

• Wastewater Treatment

• Oil Spill Cleanup (using microbes)

• Monitoring Biodiversity with DNA

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Chapter 9: Careers in Biotechnology

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• Lab Technician

• Genetic Counselor

• Biomedical Engineer

• Biotech Researcher

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Chapter 10: Ethics and the Future of Biotechnology

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• Designer Babies Debate

• Gene Editing Laws

• Ownership of Genetic Info

• Future Applications and Risks